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Also known as
CD81
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Other names
CD81 antigen; CD81 antigen; CD81 antigen; tspan-28; tetraspanin-28; 26 kDa cell surface protein TAPA-1; CD81 antigen (target of antiproliferative antibody 1); CD81 molecule; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CD81
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Gene name synonims
N/A
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Other gene names
CD81; CD81; S5.7; CVID6; TAPA1; TSPAN28; TAPA1; TSPAN28; Tspan-28
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Clonality
Monoclonal
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Immunoglobulin isotype
Hamster IgG
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Clone
EAT 2
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Host organism
Hamster
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Species reactivity
Mouse (Mus musculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
CD81 (TAPA-1) (Mouse CD81 (TAPA-1)); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.)
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Concentration
Antibody Concentration: 0.1 miligram per 1 mililiter
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Storage and shipping
Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
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Tested applications
Immunohistochemistry (IHC)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbol
CD81-AS1, CD81
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Short name
Anti-Mouse/ CD81, (Clone Eat2) (mouse IgG1)
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Technique
anti-, Mouse, anti, antibody to, antibodies, mouses
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Host
mouse
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Isotype
IgG1
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Label
Biotin
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Species
Mouse, Mouses, Rats
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Alternative name
Anti-Anti-Mouse/Rat CD81, Biotin (Clone Eat2) (mouse IgG1)
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Alternative technique
murine, antibodies
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Alternative to gene target
CD81 molecule, CVID6 and S5.7 and TAPA1 and TSPAN28, CD81 and IDBG-22455 and ENSG00000110651 and 975, MHC class II protein complex binding, Cell surfaces, Cd81 and IDBG-212677 and ENSMUSG00000037706 and 12520, CD81 and IDBG-645214 and ENSBTAG00000047495 and 511435
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Clone name
Eat2
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