Anti-Mouse IgG, HRP-Linked Antibody
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Catalog number6402-05
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PricePlease ask
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Size500 µl
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Antibody TargetAnti-Mouse IgG-HRP
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Target Alternative NameAnti-Mouse IgG, HRP-Linked Antibody, HRP linked secondary antibody
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Antibody TypePolyclonal
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Species ReactivitiesMouse
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation0.5 ml in 0.01 M PBS, pH 7.2, containing 1% BSA, 50% glycerol, 0.05% sodium azide
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PurificationThis antibody has been prepared from antisera by immunoaffinity chromatography using antigens coupled to agarose beads
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ApplicationWB 1:5000 - 1:20,000 IHC 1:500 - 1:1000 IC 1:500 - 1:1000 ELISA 1:5000 - 1:20,000 The optimal conditions should be determined individually.
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Application And UsagesWestern blotting: 1:2,000-1:100,000. Enzyme immunoassays: 1:2,000-1:100,000. Immunohistochemistry: 1:200-1:2,000. The optimal conditions should be determined individually.
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DescriptionRabbit anti-mouse IgG (H+L), horseradish Peroxidase (HPR) conjμgated. This antibody has been optimized as secondary antibody in chemiluminescent Western blotting applications
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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Additional descriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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ConjugationHRP Conjugated
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolILK, XGY1
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Short nameAnti-Mouse IgG, -Linked Antibody
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TechniqueAntibody, anti-, Mouse, anti, IgG, antibody to, antibodies, antibodies against human proteins, antibodies for, IgGs, mouses
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Hostmouse
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IsotypeRabbit IgG, IgG
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LabelHRP
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SpeciesMouse, Mouses
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Alternative nameAntibody toMouse Immunoglobulin G, horseradish peroxidase-Linked (antibody to-)
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Alternative techniqueantibodies, murine, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene nameintegrin linked kinase
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Synonyms gene name
- integrin-linked kinase
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GenBank acession
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Locus
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Discovery year1997-05-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ankyrin repeat domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameXG pseudogene, Y-linked 1
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Synonyms gene
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Synonyms gene name
- Xg pseudogene (Y-linked)
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Synonyms
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Locus
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Discovery year1995-12-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data