anti-MLF1IP Antibody

• Catalog number
  49-581
• Price
  Please ask
• Size
  0.05 mg

• Gene info
  Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/ anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.
• Gene names
  MLF1IP, CENP-U, CENP50, CENPU50, Centromere protein U, ICEN24, KLIP1, Polo-box-interacting protein 1, MLF1-interacting protein, CENP-50, CENPU, Centromere protein of 50 kDa, MLF1 interacting protein, PBIP1
• Antibodie s label
  unlabelled serum
• Purity
  Immunoaffinity Chromatography
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Spicific to
  pThr78
• Antigen
  MLF1IP antibody was made against amino acids from the C-Terminus of MLF1IP (Human).
• Immuno application
  ELISA, Western Blot, immuno-histo-chemistry, immuno-cyto-chemistry, immuno precipitation
• Antibody advice
  MLF1immunoprecipitation antibody can be used in ELISA, and immunohistochemistry starting at 2.5 ug/mL.
• Suspention pH azide
  0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Keep at
  Store MLF1IP antibody at -20 grades C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 grades C or below. As with all antibodies avoid freeze/thaw cycles.
• Works with
  Bovine, Chimpanzee, Dog, Human
• Omim nr
  74712714
• Ncbi nr
  Q71F23
• Vial with antibody
  in solution
• NCBI GENE nr
  79682
• GENE symbol
  MLF1IP
• Antigene name
  centromere protein U
• Source
  Homo sapiens
• Protein nr
  Q71F23
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MLF1IP  
• Gene symbol
  CENPU
• Short name
  anti-MLF1IP Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toMLF1IP (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:21348
• Gene
  CENPU
• Long gene name
  centromere protein U
• Synonyms gene
  • MLF1IP
• Synonyms gene name
  • MLF1 interacting protein
• Synonyms
  • CENP-U
  • KLIP1
  • CENP-50
  • PBIP1
• Synonyms name
  • polo-box-interacting protein 1
• GenBank acession
  • AK027121
  • AF516710
• Locus
  4q35.1
• Discovery year
  2003-07-17
• Entrez gene record
  79682
• Pubmed identfication
  • 15116101
  • 16622420
  • 16622419
• RefSeq identity
  • NM_024629
• Classification
  • Constitutive centromere associated network
• VEGA ID
  OTTHUMG00000160499

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products