Anti-MHC class I (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-10251R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  MHC class I
• Specificity
  This antibody reacts specifically with MHC class I
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Extracellular
• Antigen Source
  KLH conjugated synthetic peptide derived from human MHC class I
• Gene ID
  N/A
• Swiss Prot
  G0ZMJ8
• Applications
  FCM, IF(IHC-P)
• Applications with corresponding dilutions
  FCM(1:20-100), IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  A 28; A 9; Aw 24; Aw 68; CLASS I HISTOCOMPATIBILITY ANTIGEN; H2 K1; H2K; HLA A; HLA-A; HLA C; HLA-C; HLA class I histocompatibility antigen A 1 alpha chain; HLA class I histocompatibility antigen A 11 alpha chain; HLA class I histocompatibility antigen A 2 alpha chain; HLA class I histocompatibility antigen A 24 alpha chain; HLA class I histocompatibility antigen A 3 alpha chain; HLA class I histocompatibility antigen A 30 alpha chain; HLA class I histocompatibility antigen A 32 alpha chain; HLA class I histocompatibility antigen A 68 alpha chain; HLAA; Major histocompatibility complex class I A; MHC class I heavy chain H2 K; 1C04_HUMAN.
• Also known as
  MHC class I Polyclonal Antibody
• Other name
  Anti-MHC class I Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  MHC   class  
• Gene symbol
  MICD, MICF, MICG
• Short name
  Anti-MHC class I
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  MHC class I Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:7093
• Gene
  MICD
• Long gene name
  MHC class I polypeptide-related sequence D (pseudogene)
• Synonyms gene name
  • MHC class I polypeptide-related sequence D
  • MHC class I polypeptide-related sequence D, pseudogene
• Synonyms
  • PERB11.4
• Locus
  6p22.1
• Discovery year
  1994-09-07
• Entrez gene record
  4279
• Pubmed identfication
  • 8022771
  • 10557312
• RefSeq identity
  • NG_015983
• VEGA ID
  OTTHUMG00000031082

Gene info

• Identity
  HGNC:16801
• Gene
  MICF
• Long gene name
  MHC class I polypeptide-related sequence F (pseudogene)
• Synonyms gene name
  • MHC class I polypeptide-related sequence F, pseudogene
• Locus
  6p22.1
• Discovery year
  2003-05-22
• Entrez gene record
  352957
• Pubmed identfication
  • 10557312
• VEGA ID
  OTTHUMG00000031085

Gene info

• Identity
  HGNC:16802
• Gene
  MICG
• Long gene name
  MHC class I polypeptide-related sequence G (pseudogene)
• Synonyms gene name
  • MHC class I polypeptide-related sequence G, pseudogene
• Locus
  6p22.1
• Discovery year
  2003-05-22
• Entrez gene record
  352967
• Pubmed identfication
  • 10557312
• VEGA ID
  OTTHUMG00000031087

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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