Mcl-1 Antibody
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Catalog number3035-100
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PricePlease ask
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Size100 µg
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Antibody TargetMcl-1
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Target Alternative NameMCL1L, mcl1/EAT, mgC1839, mgC104264, TM, MCL1S, EAT
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat, monkey
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Immunogen SequenceSynthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1
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AccessionQ07820
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Gene Id4170
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AppearanceColorless liquid
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FormLiquid
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Concentration0.2 mg/ml
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Formulation100 µg (0.2 mg/ml) affinity purified rabbit anti-Mcl-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation, Immunohistochemistry
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Application And UsagesWestern blot analysis (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.
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DescriptionMcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-β1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMCL1
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Short nameMcl-1 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameMcl-1 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameMCL1 apoptosis regulator, BCL2 family member
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Synonyms gene name
- myeloid cell leukemia sequence 1 (BCL2-related)
- myeloid cell leukemia 1
- MCL1, BCL2 family apoptosis regulator
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-11-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- BCL2 family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data