Mcl-1 Antibody

  • Catalog number
    3035-100
  • Price
    Please ask
  • Size
    100 µg
  • Antibody Target
    Mcl-1
  • Target Alternative Name
    MCL1L, mcl1/EAT, mgC1839, mgC104264, TM, MCL1S, EAT
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human, mouse, rat, monkey
  • Immunogen Sequence
    Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1
  • Accession
    Q07820
  • Gene Id
    4170
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Concentration
    0.2 mg/ml
  • Formulation
    100 µg (0.2 mg/ml) affinity purified rabbit anti-Mcl-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
  • Purification
    Affinity purified
  • Application
    Western blot, Immunoprecipitation, Immunohistochemistry
  • Application And Usages
    Western blot analysis (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.
  • Description
    Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-β1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    Mcl-1  
  • Gene symbol
    MCL1
  • Short name
    Mcl-1 Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    Mcl-1 (antibody to-)
  • Alternative technique
    antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    MCL1 apoptosis regulator, BCL2 family member
  • Synonyms gene name
    • myeloid cell leukemia sequence 1 (BCL2-related)
    • myeloid cell leukemia 1
    • MCL1, BCL2 family apoptosis regulator
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1993-11-09
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • BCL2 family
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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