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Antibody Target
MC4 Receptor
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Target Alternative Name
melanocortin receptor 4, melanocortin 4 receptor, melanocortin-4 receptor
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Antibody Type
Polyclonal
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Species Reactivities
Human, mouse, rat
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Immunogen Sequence
Synthetic peptide surrounding amino acid 301 of mouse MC4 Receptor
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Accession
NP_058673
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Gene Id
17202
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
0.5 mg/ml
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Formulation
100 µg (0.5 mg/ml) affinity purified rabbit anti-MC4 Receptor polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal
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Purification
Affinity purified
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Application
Western blot
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Positive Control
3T3 cell lysate
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Application And Usages
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. 3T3 cell lysate can be used as a positive control.
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Description
MC4R (Melanocortin-4 receptor) belongs to the GPCR superfamily that is responsible for multiple signal transduction pathway which includes the cAMP and MAPK signaling pathways. Expression of MC4R has been reported in brain. From genetic studies of mice and humans, it was established that MC4 receptor plays a critical role in appetite regulation. Mutation in the MC4R gene is associated with obesity in humans.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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French translation
anticorps