MBD2a Antibody

• Catalog number
  3232-30T
• Price
  Please ask
• Size
  30 µg

• Antibody Target
  MBD2a
• Target Alternative Name
  Methyl-CpG-binding domain protein 2
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide surrounding amino acid 164 of rat MBD2a
• Accession
  B0BNM2
• Gene Id
  680172
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  30 µg (0.5 mg/ml) affinity purified rabbit anti- MBD2a polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Positive Control
  Jurkat cell lysate
• Application And Usages
  The antibody can be used in Western Blot analysis (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 3232BP-50) is available separately.
• Description
  The MBD family of proteins includes MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD2 is highly similar to MBD3 but only MBD2 targets the MeCP1 complex to methylate DNA. MBD2 has two isoforms, MBD2a and MBD2b. MBD2a is a 414 amino acids protein, whereas MBD2b lacks the 152 amino acids N-terminal extension. MBD2a interacts with RNA helicase A to enhance CREB-dependent gene exptression.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MBD2a  
• Short name
  MBD2a Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  MBD2a (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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