MBD1 polyclonal antibody

• Catalog number
  6828-25
• Price
  Please ask
• Size
  25 μg

• Antibody Target
  MBD1
• Target Alternative Name
  CXXC3, PCM1, RFT, Methyl-CpG-Binding Domain 1
• Antibody Type
  Polyclonal
• Species Reactivities
  Human.
• Immunogen Sequence
  MBD1 (Methyl-CpG-binding domain protein 1) synthetic peptide containing a sequence from the N-terminal conjugated to KLH
• Accession
  Q9UIS9
• Gene Id
  4152
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS with 0.05% sodium azide and 0.05% ProClin 300.
• Purification
  Affinity Purified
• Application
  Western blot, ELISA, ChIP.
• Positive Control
  Western blot: Hela cells, ELISA: peptides, ChIP: U2OS cells.
• Application And Usages
  ChIP: 1.5 µg/ChIP, WB: 1:500, ELISA: 1:1000.
• Description
  MBD1 is a transcriptional repressor that specifically binds to methylated CpG dinucleotides in promoter sequences. MBD1 acts by recruiting a variety of histone deacetylases (HDAC’s) and chromatin remodelling factors. MBD1-dependent transcriptional repression is mediated by ATF7IP through the recruitment of factors such as the histone methyltransferase SETDB1. MBD1 probably forms a complex with SETDB1 and ATF7IP which couples DNA methylation to H3K9 trimethylation and represses transcription.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Verified
• ChIP Verified
  Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  MBD1  
• Gene symbol
  MBD1
• Short name
  MBD1 polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Rabbit IgG
• Alternative name
  MBD1 polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:6916
• Gene
  MBD1
• Long gene name
  methyl-CpG binding domain protein 1
• Synonyms
  • PCM1
  • CXXC3
• GenBank acession
  • Y10746
• Locus
  18q21.1
• Discovery year
  1997-10-29
• Entrez gene record
  4152
• Pubmed identfication
  • 9207790
  • 10441743
• RefSeq identity
  • NM_015846
• Classification
  • Methyl-CpG binding domain containing
  • Zinc fingers CXXC-type
• VEGA ID
  OTTHUMG00000132669

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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