-
Gene info
MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.
-
Gene names
MBD1, RFT, PCM1, CXXC3
-
Antibodie s label
unlabelled serum
-
Purity
Antibody is purified by protein A chromatography method.
-
Ab raised in
Rabbit
-
Type
Polyclonal
-
Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MBD1.
-
Immuno application
ELISA, Western Blot
-
Antibody advice
MBD1 antibody can be used for detection of MBD1 by ELISA at 1:312500. MBD1 antibody can be used for detection of MBD1 by western blot at 1.25 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
-
Suspention pH azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
-
Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store MBD1 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
-
Works with
Human, Dog
-
Omim nr
156105673
-
Ncbi nr
NP_056669
-
KDa
60 kDa
-
Vial with antibody
Lyophilized
-
Quantity per ml
1 mg/ml
-
NCBI GENE nr
4152
-
GENE symbol
MBD1
-
Antigene name
methyl-CpG binding domain protein 1
-
Source
Homo sapiens
-
Protein nr
Q9UIS9-3
-
-
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
