Anti-Collagen VII (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-1558R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Collagen VII
• Specificity
  This antibody reacts specifically with Collagen VII
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic peptide derived from human Collagen VII
• Gene ID
  1294
• Swiss Prot
  Q02388
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  EBD1; EBR1; EBDCT; Collagen alpha-1(VII) chain; Long-chain collagen; LC collagen; COL7A1
• Also known as
  Collagen VII Polyclonal Antibody
• Other name
  Anti-Collagen VII Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Gene
  Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Collagen   VII  
• Gene symbol
  MARCHF7, IGLVVII-41-1, SYT7
• Short name
  Anti-Collagen VII
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  Collagen VII Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:17393
• Gene
  MARCHF7
• Long gene name
  membrane associated ring-CH-type finger 7
• Synonyms gene
  • AXOT
  • MARCH7
• Synonyms gene name
  • axotrophin
  • membrane-associated ring finger (C3HC4) 7
  • membrane-associated ring finger (C3HC4) 7, E3 ubiquitin protein ligase
• Synonyms
  • MARCH-VII
  • RNF177
• GenBank acession
  • AK022973
• Locus
  2q24.2
• Discovery year
  2001-12-17
• Entrez gene record
  64844
• Pubmed identfication
  • 14722266
• RefSeq identity
  • NM_022826
• Classification
  • Ring finger proteins
  • Membrane associated ring-CH-type fingers
• VEGA ID
  OTTHUMG00000132029

Gene info

• Identity
  HGNC:15691
• Gene
  IGLVVII-41-1
• Long gene name
  immunoglobulin lambda variable (VII)-41-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin lambda variable (VII)-41-1
  • immunoglobulin lambda variable (VII)-41-1 pseudogene
• Synonyms
  • IGLV(VII)-41-1
• GenBank acession
  • X99568
• Locus
  22q11.22
• Discovery year
  2001-05-15
• Entrez gene record
  84089
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151296

Gene info

• Identity
  HGNC:11514
• Gene
  SYT7
• Long gene name
  synaptotagmin 7
• Synonyms gene
  • PCANAP7
• Synonyms gene name
  • prostate cancer associated protein 7
  • synaptotagmin VII
• Synonyms
  • IPCA-7
  • SYT-VII
  • MGC150517
• GenBank acession
  • AF038535
• Locus
  11q12.2
• Discovery year
  1999-07-14
• Entrez gene record
  9066
• Pubmed identfication
  • 9615227
• RefSeq identity
  • NM_004200
• Classification
  • Synaptotagmins
• VEGA ID
  OTTHUMG00000168199

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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