LC3 (APG8C) Antibody
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Catalog number6950-100
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PricePlease ask
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Size100 µl
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Antibody TargetLC3
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Target Alternative NameMAP1LC3C; Microtubule-associated proteins 1A/1B light chain 3C; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; Microtubule-associated protein 1 light chain 3 gamma
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Antibody TypePolyclonal
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Species ReactivitiesHuman.
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Immunogen SequenceThis LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
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AccessionQ9GZQ8
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Gene Id81631
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AppearanceColorless liquid
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FormLiquid
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FormulationSupplied in PBS with 0.09% (W/V) sodium azide.
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PurificationAmmonium sulphate precipitated and dialyzed Antibody
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ApplicationWestern blot, Immunohistochemistry
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Positive ControlWB: Hela, HepG2 cells, IHC: human cancer tissue
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Application And UsagesWB: ~1:1000, IHC: 1:50-100.
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DescriptionAutophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMAP1LC3A
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Short nameLC3 (APG8C) Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameLC3 (APG8C) (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namemicrotubule associated protein 1 light chain 3 alpha
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Synonyms
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Locus
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Discovery year2000-06-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Autophagy related
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data