Anti-Nephrin (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-10233R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideNephrin
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SpecificityThis antibody reacts specifically with Nephrin
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsExtracellular
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Antigen SourceKLH conjugated synthetic peptide derived from human Nephrin
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Gene ID4868
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Swiss ProtO60500
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationSeems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsCNF; NPHN; nephrin; Renal glomerulus-specific cell adhesion receptor; NPHS1
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Also known asNephrin Polyclonal Antibody
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Other nameAnti-Nephrin Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolNPHS1, KIRREL1, KIRREL3
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Short nameAnti-Nephrin
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameNephrin Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene nameNPHS1 adhesion molecule, nephrin
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Synonyms gene name
- nephrosis 1, congenital, Finnish type (nephrin)
- NPHS1, nephrin
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Synonyms
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Locus
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Discovery year1994-12-14
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Entrez gene record
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Pubmed identfication
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Classification
- Fibronectin type III domain containing
- C2-set domain containing
- V-set domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namekirre like nephrin family adhesion molecule 1
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Synonyms gene
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Synonyms gene name
- kin of IRRE like (Drosophila)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2-set domain containing
- I-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namekirre like nephrin family adhesion molecule 3
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Synonyms gene name
- kin of IRRE like 3 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-10-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2-set domain containing
- I-set domain containing
- MicroRNA protein coding host genes
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data