Anti-Ki-67 (Proliferation Marker), ALEXA Fluor 594
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Catalog numberGENTObs-2130R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideKi-67 proliferation Marker
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SpecificityThis antibody reacts specifically with Ki-67 (Proliferation Marker)
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsNucleus
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Antigen SourceKLH conjugated synthetic peptide derived from human Ki67
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Gene ID4288
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Swiss ProtP46013
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ApplicationsIF(IHC-P), IF(ICC)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationProliferation Marker Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsKIA; MIB-; MIB-1; PPP1R15; Antigen KI-67; MKI67
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Also known asKi-67 (Proliferation Marker) Antibody
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Other nameAnti-Ki-67 (Proliferation Marker)
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Gene target
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Gene symbolMKI67P1, MKI67
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Short nameAnti-Ki-67 (Proliferation Marker)
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Techniqueanti-, anti, antibody to, antibodies, Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameKi-67 (Proliferation Marker) Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namemarker of proliferation Ki-67 pseudogene 1
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Synonyms gene name
- antigen identified by monoclonal antibody Ki-67 pseudogene 1
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Locus
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Discovery year2013-10-10
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemarker of proliferation Ki-67
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Synonyms gene name
- antigen identified by monoclonal antibody Ki-67
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-05-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data