Anti-KDEL (ER Marker) (Polyclonal), ALEXA Fluor 594
Anti-KDEL (ER Marker) (Polyclonal), ALEXA Fluor 594 is available 1 time from Bioss polyclonal antibodies labs
GENTObs-6940R-A594 | Anti-KDEL (ER Marker) (Polyclonal), ALEXA Fluor 594size: 100 microliters | 575.02 USD
- Catalog numberGENTObs-6940R-A594
- Price575.02 USD
- Size100 microliters
- ConceptScope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
- Tree numbers
- Gene ontology - Biological process
- Gene ontology - Cellular component
- Gene ontology - Molecular function
- Entrez Gene
- TypeConjugated Primary Antibody
- Conjugated withALEXA FLUOR® 594
- Host organismRabbit (Oryctolagus cuniculus)
- Target Protein/PeptideKDEL ER Marker
- SpecificityThis antibody reacts specifically with KDEL (ER Marker)
- ModificationNo modification has been applied to this antibody
- Modification siteNone
- ClonalityPolyclonal Antibody
- ClonePolyclonal Antibodies
- Concentration1ug per 1ul
- Subcellular locationsN/A
- Antigen SourceKLH conjugated synthetic peptide KDEL
- Gene IDN/A
- Swiss ProtN/A
- Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
- Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus)
- Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
- Background informationEndoplasmic reticulum Marker. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
- Purification methodPurified by Protein A.
- StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
- Excitation emission590nm/617nm
- SynonymsKDEL; Lys Asp Glu Leu antibody; GRP 78; GRP 94; KDEL1_HUMAN.
- Also known asKDEL (ER Marker) Polyclonal Antibody
- Other nameAnti-KDEL (ER Marker) Polyclonal
- AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
- PropertiesFor facs or microscopy Alexa 1 conjugate.
- ConjugationAlexa Fluor,ALEXA FLUOR® 594
- ConjugatedAlexa conjugate 1
- DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
- GroupPolyclonals and antibodies
- AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
- Gene target
- Short nameAnti-KDEL (ER ) (Polyclonal)
- TechniquePolyclonal, anti
- LabelALEXA FLUOR 594
- Alternative nameantibody to-KDEL (ER detection labelled) (polyclonal), ALEXA Fluor 594
- Alternative techniquepolyclonals
Supplier:Brady Benelux NL