JMJD2A Antibody
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Catalog number6108-100
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PricePlease ask
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Size100 μl
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Antibody TargetJMJD2A
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Target Alternative NameJumonji Domain Containing 2A; KDM4A; Lysine-Specific Demethylase 4A
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Antibody TypePolyclonal
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Species ReactivitiesHuman JMJD2A. Expected to cross react with JMJD2C (>90% similarity), JMJD2B (>85% similarity) due to high homology to these JMJD2 paralogs considering the antigen used. Observed detection of purified recombinant human JMJD2D at 50 ng sμggesting only ~10% cross reactivity with recombinant JMJD2D, however bands were detected at 60 kDa from cell samples.
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Immunogen SequenceHuman recombinant JMJD2A aa 1-350
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AccessionO75164
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Gene Id9682
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µl of antibody in TBS, pH 7.4, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide.
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PurificationAntigen affinity purified
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ApplicationWestern blot
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Positive ControlWestern blot: DLD1 cell lysate
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Application And UsagesWestern blot: 1:200 dilution. Not recommended for FACS and IHC based on probable cross reactivity with other JMJD containing proteins.
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DescriptionJumonji Domain containing 2A (JMJD2A) is a lysine specific demethylase with emerging roles in histone modification or epigenetic remodeling. This JMJD2A polyclonal antibody was raised against an N-terminal recombinant fragment of JMJD2A. This fragment includes amino acids 1-350 including the JMJN and JMJC domains but not the two LAP/PHD zinc finger or Tudor domains of the 1,064 amino acid protein. JMJD2A is detected at 135 kDa by western blotting using a DLD1 cell lysate as a positive control. Also, the expression of JMJD2A varies by tissue, and northern blotting sμggests skeletal muscle as a negative control and lung as a positive control tissue.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolKDM4A
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Short nameJMJD2A Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameJMJD2A (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namelysine demethylase 4A
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Synonyms gene
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Synonyms gene name
- jumonji domain containing 2
- jumonji domain containing 2A
- lysine (K)-specific demethylase 4A
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-08-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tudor domain containing
- Lysine demethylases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data