Hsp70 Antibody

• Catalog number
  3097-30T
• Price
  Please ask
• Size
  30 µg

• Antibody Target
  Hsp70
• Target Alternative Name
  HSPA4, APG2, APG-2, HSP70RY, HS24/P52, mgC131852 , hsp70RY
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat, monkey, hamster, rabbit, bovine
• Immunogen Sequence
  Synthetic peptide surrounding amino acid 559 of human Hsp70
• Accession
  P5462
• Gene Id
  3306
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  30 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 1% BSA, 0.02% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blot analysis (1-2 µg/ml). However, the optimal conditions should be determined individually. The antibody is specific for the inducible form of Hsp70 (Hsp72) and does not cross-react with the constitutive Hsc70 (Hsc73).
• Description
  Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but are localized within different intracellular compartments. These include: a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the highly stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in the cell experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is quite useful in ascertaining whether a stress response has occurred in the cell. It should be noted, however, that there is a constitutive level of Hsp70 expression in some cell types such as primates, e.g., human and monkey. Nevertheless increased expression of Hsp70 is observed in such cells following stress.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Hsp70  
• Gene symbol
  HSPA14, ST13, ST13P1
• Short name
  Hsp70 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Hsp70 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:29526
• Gene
  HSPA14
• Long gene name
  heat shock protein family A (Hsp70) member 14
• Synonyms gene name
  • heat shock 70kDa protein 14
• Synonyms
  • HSP70-4
  • HSP70L1
• GenBank acession
  • AF112210
• Locus
  10p13
• Discovery year
  2004-04-20
• Entrez gene record
  51182
• Pubmed identfication
  • 21208456
• RefSeq identity
  • NM_016299
• Classification
  • Heat shock 70kDa proteins

Gene info

• Identity
  HGNC:11343
• Gene
  ST13
• Long gene name
  ST13 Hsp70 interacting protein
• Synonyms gene name
  • suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
  • ST13, Hsp70 interacting protein
• Synonyms
  • SNC6
  • HSPABP1
  • HIP
  • P48
  • FAM10A1
• Synonyms name
  • progesterone receptor-associated p48 protein
• Locus
  22q13.2
• Discovery year
  1998-05-05
• Entrez gene record
  6767
• Pubmed identfication
  • 9925927
  • 8721986
• RefSeq identity
  • NM_003932
• Classification
  • Tetratricopeptide repeat domain containing
• VEGA ID
  OTTHUMG00000151201

Gene info

• Identity
  HGNC:38715
• Gene
  ST13P1
• Long gene name
  ST13, Hsp70 interacting protein pseudogene 1
• Synonyms gene name
  • suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) pseudogene 1
• Locus
  2p25.1
• Discovery year
  2010-08-11
• Entrez gene record
  729992
• RefSeq identity
  • XR_038318

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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