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Gene info
The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1 (HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1 (HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response.
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Gene names
HSBP1, DKFZp686D1664, DKFZp686O24200, NPC-A-13
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Antibodie s label
unlabelled serum
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Purity
Antibody is purified by peptide affinity chromatography method.
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSBP1.
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Immuno application
ELISA, Western Blot
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Antibody advice
HSBP1 antibody can be used for detection of HSBP1 by ELISA at 1:312500. HSBP1 antibody can be used for detection of HSBP1 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store HSBP1 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works with
Human
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Omim nr
4557647
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Ncbi nr
NP_001528
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KDa
8 kDa
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Vial with antibody
Lyophilized
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Quantity per ml
1 mg/ml
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NCBI GENE nr
3281
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GENE symbol
HSBP1
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Antigene name
heat shock factor binding protein 1
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Source
Homo sapiens
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Protein nr
O75506
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
