Histone H4 Antibody

• Catalog number
  3624-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Histone H4
• Target Alternative Name
  H4FB , HIST1H4B , HIST1H4F, H4FN, H4FH
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide corresponding to the N-terminus of human Histone H4
• Accession
  CAB02549
• Gene Id
  8367
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) affinity purified rabbit anti-Histone H4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot, Immunoprecipitation, Immunohistochemistry
• Application And Usages
  Western blotting (0.5-4 µg/ml), in immunoprecipitation (20-40 µg/ml) and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~11 kDa histone H4 protein. It does not cross-react with other histones.
• Description
  The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Histone  
• Gene symbol
  H4C8, H4-16, H4C14
• Short name
  Histone H4 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Histone H4 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:4788
• Gene
  H4C8
• Long gene name
  H4 clustered histone 8
• Synonyms gene
  • H4FH
  • HIST1H4H
• Synonyms gene name
  • H4 histone family, member H
  • histone 1, H4h
  • histone cluster 1, H4h
  • histone cluster 1 H4 family member h
• Synonyms
  • H4/h
• GenBank acession
  • X60487
• Locus
  6p22.2
• Discovery year
  1998-09-07
• Entrez gene record
  8365
• Pubmed identfication
  • 9119399
  • 12408966
• RefSeq identity
  • NM_003543
• Classification
  • H4 histones
• VEGA ID
  OTTHUMG00000014454

Gene info

• Identity
  HGNC:20510
• Gene
  H4-16
• Long gene name
  H4 histone 16
• Synonyms gene
  • HIST4H4
• Synonyms gene name
  • histone 4, H4
  • histone cluster 4, H4
  • histone cluster 4 H4
• Synonyms
  • MGC24116
• GenBank acession
  • AY128653
• Locus
  12p12.3
• Discovery year
  2003-02-20
• Entrez gene record
  121504
• Pubmed identfication
  • 12408966
• RefSeq identity
  • NM_175054
• Classification
  • H4 histones
• VEGA ID
  OTTHUMG00000168735

Gene info

• Identity
  HGNC:4794
• Gene
  H4C14
• Long gene name
  H4 clustered histone 14
• Synonyms gene
  • H4F2
  • H4FN
  • HIST2H4
  • H4/n
  • HIST2H4A
• Synonyms gene name
  • H4 histone, family 2
  • histone 2, H4
  • histone 2, H4a
  • histone cluster 2, H4a
  • histone cluster 2 H4 family member a
• GenBank acession
  • M16707
• Locus
  1q21.2
• Discovery year
  1998-09-07
• Entrez gene record
  8370
• Pubmed identfication
  • 3035717
  • 6314274
  • 15527963
• Classification
  • H4 histones
• VEGA ID
  OTTHUMG00000012189

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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