PARP Antibody (Clone 10H)
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Catalog number3000-100
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PricePlease ask
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Size100 µg
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Antibody TargetPARP
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Target Alternative NamePARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1
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Antibody TypeMonoclonal
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Clone10H
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Species ReactivitiesAll species
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AccessionNP_001609
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Gene Id142
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AppearanceColorless liquid
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FormLiquid
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Concentration1.0 mg/ml
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Formulation1 mg/ml of IgG purified from mouse ascites by protein A chromatography. Prepared in 20 mM Tris, pH 7.4, 150 mM NaCl, 1% BSA and 0.02% sodium azide.
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PurificationChromatography
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ApplicationWestern blot, Immunoprecipitation, Immunocytochemistry & ELISA
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Positive ControlTested by ICC in rat liver tissues
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Application And UsagesImmunocytochemistry : 5-20 μg/ml; Western Blot (10 μg/ml using colorimetric methods;
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DescriptionPARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedNot Verified
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Immunocytochemistry VerifiedVerified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolTNKS2, TIPARP, PARP1, PARP12, TNKS
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Short namePARP Antibody (Clone 10H)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeMouse IgG3κ
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Alternative namePARP (antibody to-) (clonality 10H)
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Alternative techniqueantibodies
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Clone name10H
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Gene info
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Identity
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Gene
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Long gene nametankyrase 2
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Synonyms gene name
- tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-05-10
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Entrez gene record
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTCDD inducible poly(ADP-ribose) polymerase
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Synonyms gene name
- TCDD-inducible poly(ADP-ribose) polymerase
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepoly(ADP-ribose) polymerase 1
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Synonyms gene
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Synonyms gene name
- ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
- poly (ADP-ribose) polymerase family, member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers PARP-type
- Zinc fingers
- Poly(ADP-ribose) polymerases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepoly(ADP-ribose) polymerase family member 12
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Synonyms gene
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Synonyms gene name
- zinc finger CCCH-type domain containing 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Zinc fingers CCCH-type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametankyrase
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Synonyms gene name
- tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-01-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Poly(ADP-ribose) polymerases
- Sterile alpha motif domain containing
- Ankyrin repeat domain containing
- MicroRNA protein coding host genes
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data