Anti-HDAC6 Monoclonal Antibody (D1 domain-specific)
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Catalog number
A1485-100
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Price
Please ask
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Size
100 µg
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Antibody Target
HDAC6
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Target Alternative Name
KIAA0901, JM21, HD6, HDAC6-D1
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Antibody Type
Monoclonal
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Clone
2H3
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Species Reactivities
Human
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Immunogen Sequence
Human Histone deacetylase 6 (HDAC6; sequence NP_006035.2, UniProtKB - Q9UBN7)
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Accession
Q9UBN7
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Gene Id
10013
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Appearance
Colorless Liquid
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Form
Liquid
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Formulation
1 mg/ml in PBS, pH 7.4, 0.02% sodium azide
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Purification
Affinity purified from hybridoma culture supernatant (Protein A)
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Application
ELISA, IC/IF
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Positive Control
HEK293T cells
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Application And Usages
ELISA: 1 – 10µg/ml; Immunocytochemistry/Immunofluorescence: 10 – 50 µg/ml
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Description
The affinity purified mouse monoclonal antibody is immunospecific for the D1 domain (a.a. 281-291) of human histone deacetylase 6 (HDAC6) as determined by ELISA and peptide library screening. HDAC6 belongs to the class II of the histone deacetylase family (EC 3.5.1.98). The protein of molecular mass 131 kDa consists of two catalytic domains, the serine-glutamate-rich SE14 domain and the ubiquitin-binding ZnF domain. HDAC6 shuttles between the nucleus and the cytoplasm, but its localization is predominantly cytoplasmic. The enzyme deacetylates various substrates including α-tubulin, HSP90, cortactin, histones and other proteins. Described protein plays an important role in microtubule-dependent cell motility, apoptosis, cell signaling and transcriptional regulation, protein degradation, immune response and DNA repair. HDAC6 is linked to several neuronal diseases and carcinogenesis
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans
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Handling
The antibody solution should be gently mixed before use
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Western Blot Verified
Not Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
1False – 5False µg/ml
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
1 – 1Falseµg/ml
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Gene symbol
HDAC6
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Short name
Anti-HDAC6 Monoclonal Antibody (D1 domain-specific)
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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Isotype
IgG
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Alternative name
Antibody toHDAC6 monoclonal (antibody to-) (D1 domain-specific)
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Alternative technique
antibodies
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Alternative to gene target
histone deacetylase 6, CPBHM and HD6 and PPP1R90, HDAC6 and IDBG-63929 and ENSG00000094631 and 10013, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, Hdac6 and IDBG-129835 and ENSMUSG00000031161 and 15185, BT.76413 and IDBG-636881 and ENSBTAG00000013244 and 513602
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Gene info
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Identity
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Gene
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Long gene name
histone deacetylase 6
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2000-11-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Histone deacetylases, class IIB
- Protein phosphatase 1 regulatory subunits
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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