Anti-HDAC6 Monoclonal Antibody (D1 domain-specific)

• Catalog number
  A1485-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  HDAC6
• Target Alternative Name
  KIAA0901, JM21, HD6, HDAC6-D1
• Antibody Type
  Monoclonal
• Clone
  2H3
• Species Reactivities
  Human
• Immunogen Sequence
  Human Histone deacetylase 6 (HDAC6; sequence NP_006035.2, UniProtKB - Q9UBN7)
• Accession
  Q9UBN7
• Gene Id
  10013
• Appearance
  Colorless Liquid
• Form
  Liquid
• Formulation
  1 mg/ml in PBS, pH 7.4, 0.02% sodium azide
• Purification
  Affinity purified from hybridoma culture supernatant (Protein A)
• Application
  ELISA, IC/IF
• Positive Control
  HEK293T cells
• Application And Usages
  ELISA: 1 – 10µg/ml; Immunocytochemistry/Immunofluorescence: 10 – 50 µg/ml
• Description
  The affinity purified mouse monoclonal antibody is immunospecific for the D1 domain (a.a. 281-291) of human histone deacetylase 6 (HDAC6) as determined by ELISA and peptide library screening. HDAC6 belongs to the class II of the histone deacetylase family (EC 3.5.1.98). The protein of molecular mass 131 kDa consists of two catalytic domains, the serine-glutamate-rich SE14 domain and the ubiquitin-binding ZnF domain. HDAC6 shuttles between the nucleus and the cytoplasm, but its localization is predominantly cytoplasmic. The enzyme deacetylates various substrates including α-tubulin, HSP90, cortactin, histones and other proteins. Described protein plays an important role in microtubule-dependent cell motility, apoptosis, cell signaling and transcriptional regulation, protein degradation, immune response and DNA repair. HDAC6 is linked to several neuronal diseases and carcinogenesis
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans
• Handling
  The antibody solution should be gently mixed before use
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  1False – 5False µg/ml
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  1 – 1Falseµg/ml
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  HDAC6   Monoclonal   domain-specific  
• Gene symbol
  HDAC6
• Short name
  Anti-HDAC6 Monoclonal Antibody (D1 domain-specific)
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Isotype
  IgG
• Alternative name
  Antibody toHDAC6 monoclonal (antibody to-) (D1 domain-specific)
• Alternative technique
  antibodies
• Alternative to gene target
  histone deacetylase 6, CPBHM and HD6 and PPP1R90, HDAC6 and IDBG-63929 and ENSG00000094631 and 10013, NAD-dependent histone deacetylase activity (H3-K18 specific), nuclei, Hdac6 and IDBG-129835 and ENSMUSG00000031161 and 15185, BT.76413 and IDBG-636881 and ENSBTAG00000013244 and 513602

Gene info

• Identity
  HGNC:14064
• Gene
  HDAC6
• Long gene name
  histone deacetylase 6
• Synonyms
  • KIAA0901
  • JM21
  • HD6
  • FLJ16239
  • PPP1R90
• Synonyms name
  • protein phosphatase 1, regulatory subunit 90
• GenBank acession
  • AF132609
• Locus
  Xp11.23
• Discovery year
  2000-11-28
• Entrez gene record
  10013
• Pubmed identfication
  • 10220385
  • 10048485
• RefSeq identity
  • NM_006044
• Classification
  • Histone deacetylases, class IIB
  • Protein phosphatase 1 regulatory subunits
• VEGA ID
  OTTHUMG00000034496
• Locus Specific Databases
  • Global Variome shared LOVD

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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