-
Type
Conjugated Primary Antibody
-
Conjugated with
ALEXA FLUOR® 594
-
Host organism
Rabbit (Oryctolagus cuniculus)
-
Target Protein Peptide
HAS3
-
Specificity
This antibody reacts specifically with HAS3
-
Modification
No modification has been applied to this antibody
-
Modification site
None
-
Clonality
Polyclonal Antibody
-
Clone
Polyclonal Antibodies
-
Concentration
1ug per 1ul
-
Subcellular locations
N/A
-
Antigen Source
KLH conjugated synthetic peptide derived from human HAS3
-
Gene ID
3038
-
Swiss Prot
N/A
-
Applications
IF(IHC-P)
-
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
-
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
-
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
-
Background information
HAS1, HAS2 and HAS3 are HA (hyaluronan or hyaluronic acid) synthase proteins. The extracellular matrix in most vertebrates express HA, which is a high molecular weight linear polysaccharide composed of alternating glucuronic acid and N-acetylglucosamine residues linked by b-1,3 and b-1,4 glycosidic bonds. The three HAS genes show distinct patterns of expression during development and their protein products play significantly different roles in the formation of the HA matrix. Both HAS1 and HAS2 synthesize high molecular-weight HA, whereas HAS3 produces lower molecular weight HA. The expression of the three HAS isoforms is more prominent in growing cells than in resting cells and is differentially regulated by various stimuli, suggesting distinct functional roles of the three proteins. HAS3 produces both secreted and cell-associated forms of hyaluronan and is the most active of the three isoforms of this enzyme in adults. HAS3 gene expression plays a crucial role in the regulation of hyaluronan synthesis in the epidermis. Specifically, IFN-g markedly upregulates HAS3 mRNA, whereas TGF Beta downregulates HAS3 transcript levels. The human HAS3 gene maps to chromosome 16q22.1.
-
Purification method
Purified by Protein A.
-
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
-
Excitation emission
590nm/617nm
-
Synonyms
HA synthase 3; HAS3; HAS 3; HAS-3; HAS3_HUMAN; Hyaluronan synthase 3; Hyaluronate synthase 3; Hyaluronic acid synthase 3.
-
Also known as
HAS3 Polyclonal Antibody
-
Other name
Anti-HAS3 Polyclonal
-
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
Properties
For facs or microscopy Alexa 1 conjugate.
-
Conjugation
Alexa Fluor, ALEXA FLUOR® 594
-
Conjugated
Alexa conjugate 1
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.