Green Fluorescence Protein (GFP) Antibody (Clone BV-F4)

• Catalog number
  3992-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  GFP
• Antibody Type
  Monoclonal
• Clone
  BV-F4
• Species Reactivities
  All species
• Immunogen Sequence
  Recombinant EGFP expressed from E. col
• Accession
  ABG78037
• Gene Id
  110612126
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) antigen affinity purified mouse anti-GFP monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (0.5-4 µg/ml), immunoprecipitation (2-8 µg/ml), immunofluorescence (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes wild-type GFP and its derivatives such as EGFP, EBFP, ECFP, and EYFP, etc. Performs well in Western blot analysis (0.5 to 4 µg/ml), immunoprecipitation, and Immunohistochemistry. However, the optimum conditions should be determined individually.
• Description
  Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can also fuse with proteins of interest without interfering significantly with their assembly and function.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Test
  Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
• French translation
  anticorps

• Gene target
  Protein   GFP   BV-F4  
• Short name
  Protein (GFP) Antibody (Clone BV-F4)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Mouse IgG1
• Label
  green
• Alternative name
  Green Fluorescence Protein (Green Fluorescent) (antibody to-) (clonality BV-F4)
• Alternative technique
  antibodies
• Clone name
  BV-F4

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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