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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 594
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
Golgin 97
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Specificity
This antibody reacts specifically with Golgin 97
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Modification
No modification has been applied to this antibody
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Modification site
None
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
N/A
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Antigen Source
KLH conjugated synthetic peptide derived from human Golgin 97
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Gene ID
2800
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Swiss Prot
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
The GRIP family member, golgin 97, is a trans-Golgi network peripheral membrane protein with an extensive coiled-coil structure (67% alpha-helical content) and a C-terminal GRIP domain. Golgin 97 localizes exclusively on the cytoplasmic face of the Golgi and can form homodimers. Binding of golgin 97 to the Golgi membrane is mediated by the G protein family member, Arl1. Golgin 97 acts as an essential player to the cell in the form of a tethering molecule associating with tubulovesicular carriers during the trafficking from the trans-Golgi network to the recycling endosome and/or early endosome. During poxvirus infection, golgin 97 accumulates at the site of viral replication and is incorporated into virions. It associates with the insoluble fraction of the virus core protein, playing a significant role in virus replication and maturation of the virus membrane and core protein. Golgin 97 takes on a rod-like shape and, although it seemingly lacks a transmembrane domain, it protrudes from the surface of the virion envelope.
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
Gap junction protein alpha 4 37kD; GOGA1_HUMAN; Golga 1; Golga1; Golgi autoantigen golgin subfamily a 1; Golgi autoantigen golgin subfamily A member 1; Golgi autoantigen golgin subfamily a1.; Golgin A1; Golgin subfamily A member 1; Golgin-97; Golgin97; MGC33154.
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Also known as
Golgin 97 Polyclonal Antibody
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Other name
Anti-Golgin 97 Polyclonal
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.