Alternative names of antibody target: Granulocyte-macrophage colony-stimulating factor; GM-CSF; GMCSF; Colony-stimulating factor; CSF; Sargramostim; Molgramostin
Background information: Murine GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages and eosinophils. Murine GM-CSF is a 14.0 kDa protein containing 124 amino acid residues.
This antibody reacts with: Mouse
Immunogene sequence: E. coli expressed murine GM-CSF
Concentration of antibody: 0.5 mg/ml
Formulation of this antibody: 30 µg (0.5 mg/ml) affinity purified rabbit anti-murine GM-CSF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.
Purification method: Affinity purified
This antibody has been succesfully tested for use in following methods: Western blot, Neutralization
Recommended usage: Western blot analysis (0.5-4 µg/ml). Recombinant murine GM-CSF (Cat.# 4101-10) can be used as a positive control. Based on researchers feedback, the antibody also works well in ELISA (2-3 µg/ml) and neutralization (5-10 µg/ml). However, the optimal conditions should be determined individually.