anti-Glycine Receptor Antibody
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Catalog number
XPS-2015
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Price
Please ask
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Size
200 ug
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Gene info
Gamma-amGlycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission. The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin. Gene targeting in mice showed that gephyrin is required for synaptic clustering of glycine receptors in spinal cord. The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins. These receptors have also been extremely useful in studies of synaptic clustering of receptors. During postnatal motoneuron development, the glycine receptor alpha subunit changes from alpha2 (fetal) to alpha1 (adult).
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Gene names
GLYRA1, Glyr, Glycine receptor 48 kDa subunit,
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Antibodie s label
unlabelled serum
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Purity
Affinity Purified
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Glycine Receptor polyclonal antibody was made against a peptide derived from the amino terminus of the *1 subunit of Glycine Receptor.
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Immuno application
Western Blot, immuno-histo-chemistry
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Antibody advice
Glycine Receptor antibody is specific immunolabeling of the ~48k *1 and *2 subunits of the receptor in Western blots of rat spinal cord and in cell extracts containing the recombinant human glycine receptors. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen derived from the amino terminus of the *1 subunit of the receptor. Applications include Dot Blots (DB) and Western Blots (WB). Rabbit anti-Glycine Receptor recognizes glycine receptor a;1 and a;2 in rat spinal cord and brain stem. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
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Keep at
Glycine Receptor antibody can be stored at -20 grades C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20 grades C. This product is stable at -20 grades C for at least 1 year.
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Works with
Rat
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Omim nr
12230887
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Ncbi nr
P07727
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KDa
48
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Vial with antibody
lyophilized
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NCBI GENE nr
25674
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GENE symbol
Glra1
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Antigene name
glycine receptor, alpha 1
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Source
Rattus norvegicus
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Protein nr
P07727
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
GLRA4, GLRB, GLRA3
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Short name
anti-Glycine Receptor Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toGlycine Receptor (antibody to-)
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Alternative technique
antibodies
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Gene info
Gene info
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Identity
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Gene
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Long gene name
glycine receptor beta
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GenBank acession
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Locus
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Discovery year
1998-08-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
glycine receptor alpha 3
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GenBank acession
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Locus
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Discovery year
1999-12-07
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Entrez gene record
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Pubmed identfication
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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