Glutathione Peroxidase 4 Antibody (Clone 1H11)
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Catalog number
6213-100
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Price
Please ask
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Size
100 μl
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Antibody Target
Glutathione Peroxidase 4
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Target Alternative Name
Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, GPX4
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Antibody Type
Monoclonal
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Clone
Clone 1H11
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Species Reactivities
Human, mouse, rat
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Immunogen Sequence
Recombinant human protein purified from E.coli
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Accession
P36969
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Gene Id
2879
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Appearance
Colorless liquid
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Form
Liquid
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Concentration
1 mg/ml
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Formulation
100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
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Purification
Ammonium sulphate precipitation
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Application
IP, ELISA.
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Positive Control
WB and IP: HL60 cell lysate
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Application And Usages
IP: 1-2 µl, ELISA.
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Description
Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic /mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
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French translation
anticorps
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Gene target
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Short name
Glutathione 4 Antibody (Clone 1H11)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Mouse IgG1
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Label
peroxidase
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Alternative name
GSH Peroxidase 4 (antibody to-) (clonality 1H11)
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Alternative technique
antibodies
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Clone name
1H11
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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