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Gene info
Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene for catalytic subunit (GLCLC) encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p21-p22. GLCLC deficiency in human is associated with enzymopathic hemolytic anemia.
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Gene names
GCLC, GCL, GCS heavy chain, Gamma-ECS, GCS, GLCL, GLCLC
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Antibodie s label
unlabelled serum
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Purity
Protein A Column
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Ab raised in
Rabbit
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Type
Polyclonal
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Spicific to
Cross-reacts weakly with bovine.
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Antigen
GCLC antibody was made against amino acids 295 - 313 of GCLC (Rat).
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Immuno application
ELISA, Western Blot, immuno-histo-chemistry, immuno precipitation
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Antibody advice
GCLC antibody can be used in immunohistochemistry starting at 20 ug/mL.
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Suspention pH azide
PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
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Keep at
Store GCLC antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
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Works with
Bovine, Human, Monkey, Mouse, Rat
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Omim nr
1346190
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Ncbi nr
P48506
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Vial with antibody
in solution
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NCBI GENE nr
2729
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GENE symbol
GCLC
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Antigene name
glutamate-cysteine ligase, catalytic subunit
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Source
Homo sapiens
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Protein nr
P48506
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
GCLC
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Short name
anti-GCLC Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toGCLC (antibody to-)
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Alternative technique
antibodies
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Alternative to gene target
glutamate-cysteine ligase, catalytic subunit, GCL and GCS and GLCL and GLCLC, GCLC and IDBG-91041 and ENSG00000001084 and 102724876,2729, coenzyme binding, Cytoplasm, Gclc and IDBG-186028 and ENSMUSG00000032350 and 14629, GCLC and IDBG-628748 and ENSBTAG00000015571 and 512468
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