Glucokinase (Human) ELISA Kit
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Catalog number
E4597-100
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Price
Please ask
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Size
100 assays
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Description
A Sandwich ELISA kit for the quantitative measurement of glucokinase in human serum, plasma, culture supernatants and other biological fluids.
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Summary
• Detection method- Absorbance (450 nm) • Species reactivity- Human • Application- quantitative measurement of glucokinase in human serum, plasma, tissue homogenates and other biological fluids.
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Detection Method
Absorbance (450 nm)
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Species Reactivity
Human
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Applications
This ELISA kit is used for quantitative measurement of glucokinase in human serum, plasma, tissue homogenates and other biological fluids.
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Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
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Features Benefits
Easy, convenient and time-saving method to measure the level of glucokinase in serum, plasma, tissue homogenates, culture supernatants and other biological fluids. Detection Range: 0.781 - 50 ng/ml Sensitivity: < 0.469 ng/ml Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10% No significant cross-reactivity or interference was observed.
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Storage Conditions
4°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Glucokinase (GCK) is expressed in specific types of tissues: liver, pancreas, small intestine and brain. Glucokinase functions as a glucose sensor, triggering shifts in carbohydrate metabolism or cell function in response to the levels of glucose in blood, such as nutritional and hormonal molecular pathways. Unlike other Hexokinases, Glucokinase has a relatively low affinity for glucose and it is not inhibited by physiological concentrations of glucose 6-phosphate. Mutations in the gene encoding GCK can cause both hyperglycemia and hypoglycemia. Due to the major role of Glucokinase in controlling blood glucose homeostasis, Glucokinase is currently considered as a strong candidate target for the treatment of Hyperglycemia, a condition encountered in Type 2 Diabetic patients. BioVision’s Glucokinase ELISA kit is a sandwich ELISA assay for the quantitative measurement of Glucokinase in Human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Glucokinase captured from the samples.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
GCKR, GCK, ADPGK
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Short name
Glucokinase ( ) ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
Glucokinase (H. sapiens) Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
glucokinase regulator
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Synonyms gene name
- glucokinase (hexokinase 4) regulatory protein
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1993-08-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
glucokinase
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Synonyms gene
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Synonyms gene name
- maturity onset diabetes of the young 2
- glucokinase (hexokinase 4)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1991-06-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Gene info
MeSH Data
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Name
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Concept
Scope note:
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
Product images
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