anti-GAPDH BIOTIN

Vial description

BIOTIN-Conjugated Antibody Glyceraldehyde-3-Phosphate dehydrogenase
Antibody s or peptide concentration

0.64-0.72 µg/µl in antibody stabilization buffer
Raised in

Rabbit
Reactivity

Human, Mouse
Immunogen

Synthetic peptide taken within amino acid region 50see msds100 on mouse Glyceraldehydesee msds3see msdsphosphate dehydrogenase protein.
Antigen or peptide

GAPDH
Original name

GAPDH BIOTIN
Phosphorylation

Non phosphorylated
Epitope

specific sequence
Type

BIOTIN-Conjugated
Antibody s relevance

ELISA, IHC, IP, WB
Antibody s application

enzyme-linked immunoabsorbant assay, EIA, immunohistochemistry, immunoprecipitation, Western Blot
Storage conditions

-20⁰C for long term storage
Genbank number

NP_032110.1
Protein number

P16858
Antibody s specifications
Antibody s techfiles
Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data