GAPDH Antibody

  • Catalog number
    3777R-100
  • Price
    Please ask
  • Size
    100 µg
  • Antibody Target
    GAPDH
  • Target Alternative Name
    GAPDH, G3PD, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human, mouse, rat
  • Immunogen Sequence
    Synthetic peptide surrounding amino acid 317of rat GADPH
  • Accession
    XP_214281.3
  • Gene Id
    290604
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Concentration
    0.5 mg/ml
  • Formulation
    100µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
  • Purification
    Affinity purified
  • Application
    Western blot
  • Application And Usages
    Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes over 100 kDa band corresponding to NIF-1 in samples from human origin. Reactivity to other species has not been tested.
  • Description
    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. While GAPDH has long been recognized as playing an integral role in glycolysis, additional functions of GAPDH include acting as a uricil DNA glycosylase, activating transcription, binding RNA and involvement in nuclear RNA export, DNA replication and DNA repair. Expression of GAPDH is upregulated in liver, lung and prostate cancers. GAPDH translocates to the nucleus during apoptosis. GAPDH complexes with neuronal proteins implicated in human neuro-degenerative disorders including the b-Amyloid precursor, Huntingtin and other triplet repeat neuronal disorder proteins.
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    GAPDH  
  • Gene symbol
    GAPDH-DT, GAPDH, GAPDHS, GAPDHP15
  • Short name
    GAPDH Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    GAPDH (antibody to-)
  • Alternative technique
    antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    GAPDH divergent transcript
  • Locus
  • Discovery year
    2021-03-12
  • Entrez gene record
  • Classification
    • Divergent transcripts
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee