anti-Gap-43 (phospho Ser41) Antibody

• Catalog number
  XPS-1011
• Price
  Please ask
• Size
  0.1 mL

• Gene info
  GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration. There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone. GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by Protein Kinase C modulates the interaction of GAP-43 with actin.
• Gene names
  Basp2, Axonal membrane protein GAP-43,
• Antibodie s label
  unlabelled serum
• Purity
  Affinity Purified
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Spicific to
  Gap-43 antibody is specific for GAP-43 phosphorylated at Ser41.
• Antigen
  GAP-43 (Ser41) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser41 of rat GAP-43.
• Immuno application
  Western Blot
• Antibody advice
  In some tissues the antibody also recognizes a higher molecular weight band that is also recognized by the Pan GAP-43 antibody and this band may be a GAP-43 aggregate or oligomer. Immunolabeling of the GAP-43 protein in Western blots of rat brain is blocked by the Ser41 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Note: the GAP-43 protein migrates on SDS-PAGE as a 50 kDa band. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot blots (DB) and Western blots (WB). Immunohistochemistry (IHC) application has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with for the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
• Suspention pH azide
  100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
• Keep at
  For long term storage -80 grades C is recommended, but shorter term storage at -20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20 grades C.
• Works with
  Human, Mouse, Rat
• Omim nr
  128102
• Ncbi nr
  P07936
• KDa
  50
• Vial with antibody
  in solution
• NCBI GENE nr
  29423
• GENE symbol
  Gap43
• Antigene name
  growth associated protein 43
• Source
  Rattus norvegicus
• Protein nr
  P07936
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Gap-43   phospho   Ser41  
• Short name
  anti-Gap-43 (phospho Ser41) Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toGap-43 (phosphorilated Ser41) (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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