anti-Follistatin Antibody
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Catalog number
38-137
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Price
Please ask
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Size
0.1 mg
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Gene info
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone
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Gene names
FSFollistatin, Activin-binding protein, FS
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Antibodie s label
unlabelled serum
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Ab raised in
Rabbit
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Type
Polyclonal
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Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Human Follistatin specific antibody was purified by affinity chromatography employing immobilized hFollistatin matrix.
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Immuno application
ELISA, Western Blot, NELISAut
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Antibody advice
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100.0 ng/mL), a concentration of 8.0-12.0 ug/mL of this antibody is required.ELISA:Indirect:To detect hFollistatin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.SandwichTo detect hFollistatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Follistatin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin. Western Blot:To detect hFollistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Keep at
Follistatin antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
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Works with
Human
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Omim nr
23831079
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Ncbi nr
P19883
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Vial with antibody
Lyophilized
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NCBI GENE nr
10468
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GENE symbol
FST
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Antigene name
follistatin
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Source
Homo sapiens
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Protein nr
P19883
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
FST, FSTL1, FSTL3
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Short name
anti-Follistatin Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative name
Antibody toFollistatin (antibody to-)
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
follistatin
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
follistatin like 1
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- SPARC family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
follistatin like 3
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Synonyms gene name
- follistatin-like 3 (secreted glycoprotein)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1999-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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