Flotillin 1 Antibody
-
Catalog numberPA2033
-
PricePlease ask
-
Size0,1 mg
-
-
Target antigenFlotillin 1
-
ClonalityPolyclonal antibody
-
ClonePolyclonal antibody
-
Raised inrabbit
-
Type of the antibodyIgG polyclonal antibody
-
Product formfreeze-dried
-
Reacts with specieshuman, rat Predicted to work with: mouse
-
AnalysesWB
-
ImmunogenA synthetic peptide corresponding to a sequence in the middle region of human Flotillin 1(219-234aa KKAAYDIEVNTRRAQA), different from the related rat and mouse sequences by one amino acid.
-
Product configurationEach vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
-
PurificationImmunogen affinity purified.
-
SolubilizationThe powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
-
Storage condtionsKeep the Flotillin 1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
-
TipsThe Flotillin 1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
-
BackgroundFLOT1(Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al.(1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al.(1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al.(2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.
-
Related articles1. Bickel, P. E., Scherer, P. E., Schnitzer, J. E., Oh, P., Lisanti, M. P., Lodish, H. F. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J. Biol. Chem. 272: 13793-13802, 1997. 2. Hazarika, P., Dham, N., Patel, P., Cho, M., Weidner, D., Goldsmith, L., Duvic, M. Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation. J. Cell. Biochem. 75: 147-159, 1999. 3. Santamaria, A., Castellanos, E., Gomez, V., Benedit, P., Renau-Piqueras, J., Morote, J., Reventos, J., Thomson, T. M., Paciucci, R. PTOV1 enables the nuclear translocation and mitogenic activity of flotillin-1, a major protein of lipid rafts. Molec. Cell. Biol. 25: 1900-1911, 2005.
-
Gene NameFLOT1
-
Protein NameFlotillin-1
-
Gene Full Nameflotillin 1
-
SynonymsFLOT 1 antibody|FLOT1 antibody|FLOT1_HUMAN antibody|Flotillin-1 antibody|Flotillin1 antibody|Integral membrane component of caveolae antibody|Reggie 2 antibody
-
Uniprot IDO75955
-
PropertiesIf you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolFLOT1, FLOT2
-
Short nameFlotillin 1 Antibody
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
Alternative nameFlotillin 1 (antibody to-)
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameflotillin 1
-
GenBank acession
-
Locus
-
Discovery year2000-05-30
-
Entrez gene record
-
Classification
- Flotillins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameflotillin 2
-
Synonyms gene
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1991-05-09
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Flotillins
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data