Anti- FLAG tag Antibody

• Catalog number
  GEN2517694
• Price
  Please ask
• Size
  0.12 ml

• Long name
  FLAG tag Polyclonal Antibody
• Antigen
  FLAG tag
• Synonim names
  DDDDK epitope tag; DYKDDDDK epitope tag
• Other names
  N/A
• Gene name
  FLAG tag ; FLAG
• Gene synonims
  N/A
• Category
  Antibodies
• Clonality
  Polyclonal Antibodies
• Clone
  Not applicable to Polyclonal Antibodies
• Reacts with
  Fusion proteins containing the Flag Tag
• Specificity
  This is an antibody designed to detect FLAG tag ; FLAG
• Purification method
  This antibody was purified via antigen affinity purification method
• Form Appearance
  N/A
• Concentration
  1.4mg/mL
• Storage shipping and handling
  The antibody is shipped at +4 degrees Celsius. Upon receving, freeze at -20. For longer periods of time we recommend keeping the vial frozen at -40 or -80. Avoid cycles of freezing and thawing as they may denaturate the polypeptide chains of the antibody. Due to transportation or handling reasons, small amounts of the antibody might get caught on the lid or walls of the vial. We recommend you to briefly cetrifuge the vial prior to use to gather the content on the bottom.
• Applications
  ELISA, EIA, IFA, ELI-Spot, Western Blot (WB)
• Clonagen antibody
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag
• French translation
  anticorps

• Gene target
  tag  
• Gene symbol
  TRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
• Short name
  Anti-FLAG tag
• Technique
  Antibody, anti, antibody to, antibodies against human proteins, antibodies for, Tags
• Host
  Rabbit (Oryctolagus cuniculus)
• Isotype
  Immunoglobulin G (IgG)
• Label
  Flag
• Alternative name
  FLAG tag Antibody
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:34713
• Gene
  TRL-TAG2-1
• Long gene name
  tRNA-Leu (anticodon TAG) 2-1
• Synonyms gene
  • TRNAL15
• Synonyms gene name
  • transfer RNA leucine 15 (anticodon UAG)
  • transfer RNA-Leu (TAG) 2-1
• Synonyms
  • tRNA-Leu-TAG-2-1
• GenBank acession
  • HG983905
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189151
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34591
• Gene
  TRL-TAG3-1
• Long gene name
  tRNA-Leu (anticodon TAG) 3-1
• Synonyms gene
  • TRNAL7
• Synonyms gene name
  • transfer RNA leucine 7 (anticodon UAG)
  • transfer RNA-Leu (TAG) 3-1
• Synonyms
  • tRNA-Leu-TAG-3-1
• GenBank acession
  • HG983906
• Locus
  16p12.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189032
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:35011
• Gene
  TRL-TAG4-1
• Long gene name
  tRNA-Leu (anticodon TAG) 4-1
• Synonyms gene
  • TRNAL40P
• Synonyms gene name
  • transfer RNA leucine 40 (anticodon UAG) pseudogene
  • transfer RNA-Leu (TAG) 4-1
• Synonyms
  • tRNA-Leu-TAG-4-1
• GenBank acession
  • HG983907
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189441
• RefSeq identity
  • NG_008497
• Classification
  • Cytoplasmic transfer RNA pseudogenes

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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