Anti-FLAG Octapeptide (FLAG) Tag Monoclonal Antibody

  • Catalog number
    CAU29725
  • Price
    Please ask
  • Size
    200 μg
  • Other name
    FLAG epitope; FLAG-Tag
  • Immunogen
    no specific immunogen / contact us
  • Verified reactivity
    Pan-species (General)
  • Verified applications
    WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
  • Raised in
    Mouse
  • Clonality
    Monoclonal
  • Protein number
    Please see Uniprot
  • Gene number
    Please refer to GenBank
  • NCBI number
    Please refer to NCBI
  • Purity
    Affinity purified. Please contact us for more details
  • Antibody s concentration
    500μg/ml
  • Stroage recommendation
    Aliquot and store at -20°C. Minimize freezing and thawing.
  • Use before
    1 year
  • Shipping requirements
    Blue ice
  • Estimated production time
    7-11 business days
  • Supplementary information
    Applicable secondary antibody is available. Please inquire
  • Notes
    For research use only. Not for diagnostic procedures.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
  • Conjugation
    Flag
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • French translation
    anticorps
  • Gene target
  • Short name
    Anti- Octapeptide ( ) Tag Monoclonal Antibody
  • Technique
    Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
  • Label
    Unconjugated. Biotin/FITC/Cy3/Cy5 labelling is available, please inquire.
  • Alternative name
    Antibody toFLAG Octapeptide (FLAG) detection labelled monoclonal (antibody to-)
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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