Anti-FLAG Octapeptide (FLAG) Tag Monoclonal Antibody
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Catalog number
CAU29725
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Price
Please ask
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Size
200 μg
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Other name
FLAG epitope; FLAG-Tag
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Immunogen
no specific immunogen / contact us
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Verified reactivity
Pan-species (General)
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Verified applications
WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
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Raised in
Mouse
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Clonality
Monoclonal
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Protein number
Please see Uniprot
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Gene number
Please refer to GenBank
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NCBI number
Please refer to NCBI
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Purity
Affinity purified. Please contact us for more details
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Antibody s concentration
500μg/ml
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Stroage recommendation
Aliquot and store at -20°C. Minimize freezing and thawing.
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Use before
1 year
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Shipping requirements
Blue ice
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Estimated production time
7-11 business days
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Supplementary information
Applicable secondary antibody is available. Please inquire
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Notes
For research use only. Not for diagnostic procedures.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Short name
Anti- Octapeptide ( ) Tag Monoclonal Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
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Label
Unconjugated. Biotin/FITC/Cy3/Cy5 labelling is available, please inquire.
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Alternative name
Antibody toFLAG Octapeptide (FLAG) detection labelled monoclonal (antibody to-)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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