Anti-Fast skeletal Myosin (Polyclonal), ALEXA Fluor 594
-
Catalog numberGENTObs-5159R-A594
-
PricePlease ask
-
Size100 microliters
-
-
TypeConjugated Primary Antibody
-
Conjugated withALEXA FLUOR® 594
-
Host organismRabbit (Oryctolagus cuniculus)
-
Target Protein PeptideFast skeletal Myosin
-
SpecificityThis antibody may recognize myosin light chain 5, myosin light chain 7, or myosin regulatory light chain 10 Fast skeletal Myosin
-
ModificationNo modification has been applied to this antibody
-
Modification siteNone
-
ClonalityPolyclonal Antibody
-
ClonePolyclonal Antibodies
-
Concentration1ug per 1ul
-
Subcellular locationsCytoplasm
-
Antigen SourceKLH conjugated synthetic peptide derived from human MRLC2
-
Gene ID29895
-
Swiss ProtQ96A32
-
ApplicationsIF(IHC-P)
-
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
-
Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
-
Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
-
Background informationMyosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins . Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.
-
Purification methodPurified by Protein A.
-
StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
-
Excitation emission590nm/617nm
-
SynonymsMRLC2; MYL11; HUMMLC2B; Myosin regulatory light chain 2, skeletal muscle isoform; Fast skeletal myosin light chain 2; MLC2B; MYLPF
-
Also known asFast skeletal Myosin Polyclonal Antibody
-
Other nameAnti-Fast skeletal Myosin Polyclonal
-
AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
PropertiesFor facs or microscopy Alexa 1 conjugate.
-
ConjugationAlexa Fluor, ALEXA FLUOR® 594
-
ConjugatedAlexa conjugate 1
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
GroupPolyclonals and antibodies
-
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
Gene target
-
Gene symbolMYL1, MYBPC2, MYLPF
-
Short nameAnti-Fast skeletal Myosin
-
TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
IsotypeImmunoglobulin G (IgG)
-
LabelALEXA FLUOR 594
-
Alternative nameFast skeletal Myosin Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
-
Alternative techniquepolyclonals, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene namemyosin light chain 1
-
Synonyms gene name
- myosin, light polypeptide 1, alkali; skeletal, fast
- myosin, light chain 1, alkali; skeletal, fast
-
Locus
-
Discovery year1986-01-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Myosin light chains, class 1
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemyosin binding protein C2
-
Synonyms gene name
- myosin-binding protein C, fast-type
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1993-12-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- I-set domain containing
- Myosin binding proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemyosin light chain, phosphorylatable, fast skeletal muscle
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2009-03-06
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Myosin light chains, class 2
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data