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Target antigen
Factor VIII
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
rat Predicted to work with: mouse
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Factor VIII(1264-1278aa APVLQDFRSLNDSTN).
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Factor VIII Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Factor VIII Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Coagulation Factor VIII(FVIII) is an essential blood-clotting protein, also known as anti-hemophilic factor(AHF). By in situ hybridization, Tantravahi et al.(1986) concluded that the F8 gene is located in the proximal part of chromosome Xq28 with probes DX13 and St14 distally located. The F8 gene encodes coagulation factor VIII, a large plasma glycoprotein that functions in the blood coagulation cascade as a cofactor for the factor IXa-dependent activation of factor X(F10). Factor VIII is activated proteolytically by a variety of coagulation enzymes, including thrombin(F2). Factor VIII is tightly associated in the blood with von Willebrand factor(VWF), which serves as a protective carrier protein for factor VIII(Toole et al., 1984; Hoyer, 1994).
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Related articles
1. Hoyer, L. W. Hemophilia A. New Eng. J. Med. 330: 38-45, 1994. 2. Tantravahi, U., Murty, V. V. V. S., Jhanwar, S. C., Toole, J. J., Woozney, J. M., Chaganti, R. S. K., Latt, S. A. Physical mapping of the factor VIII gene proximal to two polymorphic DNA probes in human chromosome band Xq28: implications for factor VIII gene segregation analysis. Cytogenet. Cell Genet. 42: 75-79, 1986. 3. Toole, J. J., Knopf, J. L., Wozney, J. M., Sultzman, L. A., Buecker, J. L., Pittman, D. D., Kaufman, R. J., Brown, E., Shoemaker, C., Orr, E. C., Amphlett, G. W., Foster, W. B., Coe, M. L., Knutson, G. J., Fass, D. N., Hewick, R. M. Molecular cloning of a cDNA encoding human antihaemophilic factor. Nature 312: 342-347, 1984.
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Gene Name
F8
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Protein Name
Coagulation factor VIII
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Gene Full Name
coagulation factor VIII, procoagulant component
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Synonyms
Ahf antibody|Antihemophilic factor antibody|Coagulation factor VIII antibody|Coagulation factor VIII associated protein b antibody|Coagulation factor VIII isoform b antibody|Coagulation factor VIII procoagulent component antibody|coagulation factor VIII, procoagulant component antibody|Coagulation factor VIIIc antibody|Dna segment on chromosome x unique 1253 expressed sequence antibody|Dxs1253e antibody|F8 antibody|F8 protein antibody|F8b antibody|F8c antibody|FA8_HUMAN antibody|Factor VIII F8b antibody|Factor VIIIa light chain antibody|FactorVIII antibody|FVIII antibody|Hema antibody|Hema coagulation factor VIIIc procoagulent component antibody|Hemophilia a antibody|Hemophilia classic antibody|Hemophilia, classic antibody|OTTHUMP00000061446 antibody|OTTHUMP00000196174 antibody|Procoagulant component antibody
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Uniprot ID
P00451
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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French translation
anticorps
