Anti-EZH1 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-6528R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  EZH1
• Specificity
  This antibody reacts specifically with EZH1
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Nucleus
• Antigen Source
  KLH conjugated synthetic peptide derived from human EZH1
• Gene ID
  2145
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. Sequence similarities Belongs to the histone-lysine methyltransferase family. EZ subfamily.Contains 1 SET domain.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  Enhancer of zeste homolog 1Drosophila; Enhancer of zeste homolog 1; ENX-2; ENX2; EZH1; EZH1: enhancer of zeste homolog 1 Drosophila; EZH1_HUMAN; Histone-lysine N-methyltransferase EZH1; KIAA0388.
• Also known as
  EZH1 Polyclonal Antibody
• Other name
  Anti-EZH1 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  EZH1  
• Gene symbol
  EZH1, EZH2
• Short name
  Anti-EZH1
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  EZH1 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:3526
• Gene
  EZH1
• Long gene name
  enhancer of zeste 1 polycomb repressive complex 2 subunit
• Synonyms gene name
  • enhancer of zeste (Drosophila) homolog 1
  • enhancer of zeste homolog 1 (Drosophila)
• Synonyms
  • KIAA0388
  • KMT6B
• Locus
  17q21.2
• Discovery year
  1995-12-21
• Entrez gene record
  2145
• Pubmed identfication
  • 8921387
• RefSeq identity
  • NM_001991
• Classification
  • SET domain containing
  • Polycomb repressive complex 2
  • Lysine methyltransferases
  • Myb/SANT domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000180645

Gene info

• Identity
  HGNC:3527
• Gene
  EZH2
• Long gene name
  enhancer of zeste 2 polycomb repressive complex 2 subunit
• Synonyms gene name
  • enhancer of zeste (Drosophila) homolog 2
  • enhancer of zeste homolog 2 (Drosophila)
• Synonyms
  • EZH1
  • ENX-1
  • KMT6
  • KMT6A
• Locus
  7q36.1
• Discovery year
  1995-12-21
• Entrez gene record
  2146
• Pubmed identfication
  • 8954776
  • 17172412
• RefSeq identity
  • NM_004456
• Classification
  • SET domain containing
  • Polycomb repressive complex 2
  • Lysine methyltransferases
  • Myb/SANT domain containing
• VEGA ID
  OTTHUMG00000158973
• Locus Specific Databases
  • LRG_531

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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