Anti-EphA8 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-7035R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  EphA8
• Specificity
  This antibody reacts specifically with EphA8
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Extracellular
• Antigen Source
  KLH conjugated synthetic peptide derived from human EphA8/Eph receptor A8
• Gene ID
  2046
• Swiss Prot
  N/A
• Applications
  FCM, IF(IHC-P)
• Applications with corresponding dilutions
  FCM(1:20-100), IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  EphA8, is a member of the Eph receptor family, which binds members of the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. EphA8 binds to Ephrin-A2, Ephrin-A3, and Ephrin-A5. Ephs and ephrins play a significant role in angiogenesis.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  EEK; EK3; EPH and ELK related kinase; EPH like kinase 3; EphA8; Ephrin Type A Receptor 8 Precursor; HEK3; KIAA1459; Tyrosylprotein Kinase; 2.7.1.112; EPHA8_HUMAN.
• Also known as
  EphA8 Polyclonal Antibody
• Other name
  Anti-EphA8 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  EphA8  
• Gene symbol
  EPHA8
• Short name
  Anti-EphA8
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  EphA8 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:3391
• Gene
  EPHA8
• Long gene name
  EPH receptor A8
• Synonyms gene
  • EEK
• Synonyms gene name
  • EphA8
• Synonyms
  • Hek3
• GenBank acession
  • BC038796
• Locus
  1p36.12
• Discovery year
  1990-09-30
• Entrez gene record
  2046
• Pubmed identfication
  • 1648701
• RefSeq identity
  • NM_020526
• Classification
  • EPH receptors
  • Sterile alpha motif domain containing
• VEGA ID
  OTTHUMG00000002892

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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