DNMT3A polyclonal antibody

• Catalog number
  6849-25
• Price
  Please ask
• Size
  25 μg

• Antibody Target
  DNMT3A
• Target Alternative Name
  DNA Methyltransferase 3a
• Antibody Type
  Polyclonal
• Species Reactivities
  Human.
• Immunogen Sequence
  Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 92-106.
• Accession
  Q9Y6K1
• Gene Id
  1788
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS with 0.05% sodium azide and 0.05% ProClin 300.
• Purification
  Affinity Purified
• Application
  Western blot, ELISA, IP.
• Positive Control
  Western blot: HEK 293 T cells, ELISA: Peptides, IP: HEK 293 T cells.
• Application And Usages
  Western Blot: 1:2,500, ELISA: 1:100, IP: 2 µg/IP.
• Description
  DNMT3A catalyzes the genome wide de novo methylation of CpG residues. DNA methylation on CpG residues by DNMT3A regulates gene expression and is essential for development. DNMT3A is strongly expressed in embryonic stem cells, but low in adult somatic cells. DNA methylation is coordinated with methylation of histones. DNMT3A binds to SETDB1 and HDAC1, and is involved in the repression of transcription from promoters containing an E2F binding site.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Verified
• ELISA Verified
  Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  DNMT3A  
• Gene symbol
  DNMT3A
• Short name
  DNMT3A polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Rabbit IgG
• Alternative name
  DNMT3A polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:2978
• Gene
  DNMT3A
• Long gene name
  DNA methyltransferase 3 alpha
• Synonyms gene name
  • DNA (cytosine-5-)-methyltransferase 3 alpha
• Locus
  2p23.3
• Discovery year
  1998-07-15
• Entrez gene record
  1788
• Pubmed identfication
  • 9662389
  • 10433969
• RefSeq identity
  • NM_022552
• Classification
  • MicroRNA protein coding host genes
  • 7BS C5-cytosine DNA/RNA methyltransferases
  • PWWP domain containing
• VEGA ID
  OTTHUMG00000094777
• Locus Specific Databases
  • LRG_459

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products