Dnmt2 Antibody

• Catalog number
  3488-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  DNMT2
• Target Alternative Name
  Dnmt2 , TRDMT1 , RNMT1 , OTTHUMP00000045198 , cytosine-5-methyltransferase , EC 2.1.1.29
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse
• Immunogen Sequence
  Synthetic peptides surrounding amino acid 42 and 364 of mouse Dnmt2
• Accession
  O55055
• Gene Id
  13434
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot, Immunofluorescence
• Application And Usages
  Western blotting (0.5-4 µg/ml) and immunofluorescence. However, the optimal concentrations should be determined individually. The antibody recognizes 42 kDa Dnmt 2 of human and mouse origins. Mouse testes tissue lysate can be use as a positive control.
• Description
  Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Dnmt2  
• Gene symbol
  TRDMT1
• Short name
  Dnmt2 Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Dnmt2 (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:2977
• Gene
  TRDMT1
• Long gene name
  tRNA aspartic acid methyltransferase 1
• Synonyms gene
  • DNMT2
• Synonyms gene name
  • DNA (cytosine-5-)-methyltransferase 2
• Synonyms
  • RNMT1
• Synonyms name
  • tRNA (cytosine(38)-C(5))-methyltransferase
• GenBank acession
  • AJ223333
• Locus
  10p13
• Discovery year
  1998-05-07
• Entrez gene record
  1787
• Pubmed identfication
  • 9425235
  • 9763678
  • 16424344
• RefSeq identity
  • NM_004412
• Classification
  • 7BS C5-cytosine DNA/RNA methyltransferases
• VEGA ID
  OTTHUMG00000017745

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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