DNA Methyltransferase 3a (Clone 64B814.1) Antibody
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Catalog number
6113-50
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Price
Please ask
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Size
50 μg
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Antibody Target
DNMT3A
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Target Alternative Name
DNMT3, DNMT3A
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Antibody Type
Monoclonal
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Clone
64B814.1
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Species Reactivities
Human, mouse
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Immunogen Sequence
Recombinant mouse His-tagged DNMT3a expressed in bacteria
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Accession
O88508
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Gene Id
13435
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
50 µg of antibody in 100 µl PBS containing 0.05% BSA and 0.05% sodium azide.
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Purification
Protein G purified
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Application
Western blot, Immunofluorescence, Immunocytochemistry
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Positive Control
Western blot: Transfected 293 cell lysate, untransfected 293 cell lysate
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Application And Usages
Western blot: 1-3 µg/ml, IF/ICC: 5 µg/ml. However, the optimal conditions should be determined individually.
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Description
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The DNMT3 family members, DNMT3a and DNMT3b, are strongly expressed in embryonic stem (ES) cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of DNMT3b gene occur in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Verified
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Immunoprecipitation Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
DNA Methyltransferase 3a (Clone 64B814 1) Antibody
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Technique
Antibody, dna, antibodies against human proteins, antibodies for
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Isotype
Mouse IgG1κ
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Alternative name
Desoxyribonucleic acid Methyltransferase 3a (clonality 64B814.1) (antibody to-)
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Alternative technique
antibodies
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Clone name
64B814
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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