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Target antigen
Diubiquitin
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Diubiquitin(27-40aa YDSVKKIKEHVRSK), different from the related mouse and rat sequences by five amino acids.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Diubiquitin Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Diubiquitin Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Ubiquitin D, also called UBD or FAT10 is a protein that in humans is encoded by the UBD gene. This gene is mapped to 6p22.1. Ubiquitin-like protein modifier can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome in a NUB1L-dependent manner. This gene may be involved in dendritic cell(DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. It may be involved in the formation of aggresomes when proteasome is saturated or impaired. This gene mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus(HIV)-associated nephropathy(HIVAN). It probably functions as a survival factor.
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Related articles
1. Bates, E. E. M., Ravel, O., Dieu, M.-C., Ho, S., Guret, C., Bridon, J.-M., Ait-Yahia, S., Briere, F., Caux, C., Banchereau, J., Lebecque, S. Identification and analysis of a novel member of the ubiquitin family expressed in dendritic cells and mature B cells. Europ. J. Immun. 27: 2471-2477, 1997. 2. Buchsbaum, S., Bercovich, B., Ciechanover, A. FAT10 is a proteasomal degradation signal that is itself regulated by ubiquitination. Molec. Biol. Cell 23: 225-232, 2012. 3. Chiu, Y.-H., Sun, Q., Chen, Z. J. E1-L2 activates both ubiquitin and FAT10. Molec. Cell 27: 1014-1023, 2007.
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Gene Name
UBD
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Protein Name
Ubiquitin D
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Gene Full Name
ubiquitin D
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Synonyms
Diubiquitin antibody|FAT10 antibody|UBD 3 antibody|Ubd antibody|UBD_HUMAN antibody|Ubiquitin D antibody|Ubiquitin like protein FAT10 antibody|Ubiquitin-like protein FAT10 antibody
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Uniprot ID
O15205
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
