DEDAF Antibody

• Catalog number
  3313-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  DEDAF
• Target Alternative Name
  DEDAF , YEAF1 , AAP1 , APAP-1, RYBP, RING1 and YY1-binding protein; Death effector domain-associated factor; DED-associated factor; YY1 and E4TF1-associated factor 1; Apoptin-associating protein 1; APAP-1
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide surrounding amino acid 218 of human DEDAF
• Accession
  Q8N488
• Gene Id
  23429
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) affinity purified rabbit anti-DEDAF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. A549 or HepG2 cell lysate can be used as a positive control. A 32 kDa band should be detected.
• Description
  Cell death signal are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. A novel protein that interacts with DED of caspase-8 and -10, and FADD was recently identified and designated DEDAF (for DED associated factor). DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and aμgments the formation of CD95/FADD/caspase-8 complexes at the cytoplasm. DEDAF also interacts with DED-containing DNA biding protein (DEDD) in the nucleus, indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  DEDAF  
• Gene symbol
  RYBP
• Short name
  DEDAF Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  DEDAF (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10480
• Gene
  RYBP
• Long gene name
  RING1 and YY1 binding protein
• Synonyms
  • YEAF1
  • AAP1
  • DEDAF
• Synonyms name
  • YY1 and E4TF1 associated factor 1
  • ring1 interactor RYBP
  • apoptin-associating protein 1
  • death effector domain-associated factor
• GenBank acession
  • AF179286
• Locus
  3p13
• Discovery year
  2000-01-28
• Entrez gene record
  23429
• Pubmed identfication
  • 10369680
• RefSeq identity
  • NM_012234
• Classification
  • Zinc fingers RANBP2-type
• VEGA ID
  OTTHUMG00000159190

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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