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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 555
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
DAND5
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Specificity
This antibody reacts specifically with DAND5 ALEXA FLUOR® 555
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Modification
No modification has been applied to this antibody
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Modification site
None
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
Extracellular, Secreted
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Antigen Source
KLH conjugated synthetic peptide derived from human DAND5
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Gene ID
199699
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Swiss Prot
Q8N907
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
Seems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, tissue growth and differentiation (By similarity).
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
553nm/568nm
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Synonyms
SP1; CER2; COCO; CRL2; CERL2; DANTE; GREM3; CKTSF1B3; DAN domain family member 5; Cerberus-like protein 2; Cerl-2; Cysteine knot superfamily 1, BMP antagonist 3; Gremlin-3; DAND5
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Also known as
DAND5 Polyclonal Antibody ALEXA FLUOR® 555
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Other name
Anti-DAND5 Polyclonal ALEXA FLUOR® 555
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate. Very high photo stable ALEXA conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR 555
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbol
DAND5
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Short name
Anti-DAND5 ALEXA FLUOR® 555
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Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 555
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Alternative name
DAND5 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
DAN domain family, member 5, DAND5 and IDBG-31601 and ENSG00000179284 and 199699, morphogen activity, Extracellular, Dand5 and IDBG-174811 and ENSMUSG00000053226 and 23863
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