D4-GDI Antibody

• Catalog number
  3385-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  D4-GDI
• Target Alternative Name
  ARHGDIB , 602843 , P52566 , D4 , GDIA2 , GDID4 , LYGDI , Ly-GDI , RAP1GN1
• Antibody Type
  Polyclonal
• Species Reactivities
  Human
• Immunogen Sequence
  Synthetic peptide near the N-terminus of human D4-GDI
• Accession
  P52566
• Gene Id
  397
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 28 kDa D4-GDI in human samples. Jurkat cell lysate can be used as a positive control.
• Description
  D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeleytal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block throμgh its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kDa and 5kDa size fragment. The 23kDa fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  D4-GDI  
• Gene symbol
  DPF2, DPF1, DPF3, ARHGDIB
• Short name
  D4-GDI Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  D4-GDI (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9964
• Gene
  DPF2
• Long gene name
  double PHD fingers 2
• Synonyms gene
  • REQ
• Synonyms gene name
  • requiem, apoptosis response zinc finger gene
  • D4, zinc and double PHD fingers family 2
• Synonyms
  • ubi-d4
  • BAF45d
  • SMARCG2
• GenBank acession
  • U94585
• Locus
  11q13.1
• Discovery year
  1997-05-15
• Entrez gene record
  5977
• Pubmed identfication
  • 11845289
• RefSeq identity
  • NM_006268
• Classification
  • BAF complex
  • PHD finger proteins
• VEGA ID
  OTTHUMG00000165985

Gene info

• Identity
  HGNC:20225
• Gene
  DPF1
• Long gene name
  double PHD fingers 1
• Synonyms gene name
  • D4, zinc and double PHD fingers family 1
• Synonyms
  • neuro-d4
  • NEUD4
  • BAF45b
  • SMARCG1
• GenBank acession
  • U43843
• Locus
  19q13.2
• Discovery year
  2003-01-28
• Entrez gene record
  8193
• Pubmed identfication
  • 8812431
• Classification
  • PHD finger proteins
  • BAF complex
• VEGA ID
  OTTHUMG00000157164

Gene info

• Identity
  HGNC:17427
• Gene
  DPF3
• Long gene name
  double PHD fingers 3
• Synonyms gene name
  • D4, zinc and double PHD fingers, family 3
• Synonyms
  • cer-d4
  • Cerd4
  • FLJ14079
  • BAF45c
  • SMARCG3
• GenBank acession
  • U43919
• Locus
  14q24.2
• Discovery year
  2003-01-28
• Entrez gene record
  8110
• Pubmed identfication
  • 11845289
  • 8812431
• Classification
  • PHD finger proteins
  • BAF complex
• VEGA ID
  OTTHUMG00000171364

Gene info

• Identity
  HGNC:679
• Gene
  ARHGDIB
• Long gene name
  Rho GDP dissociation inhibitor beta
• Synonyms gene
  • RAP1GN1
  • GDIA2
  • GDID4
• Synonyms gene name
  • Rho GDP dissociation inhibitor (GDI) beta
• Synonyms
  • Ly-GDI
  • RhoGDI2
• GenBank acession
  • L07916
• Locus
  12p12.3
• Discovery year
  1994-08-29
• Entrez gene record
  397
• Pubmed identfication
  • 8434008
  • 8356058
• RefSeq identity
  • NM_001175
• Classification
  • Receptor ligands
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000168742

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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