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Target antigen
CYP7A1
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human Predicted to work with: mouse, rat
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Analyses
WB,IHC-P
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CYP7A1(194-208aa RRDTQKAHILNNLDN).
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the CYP7A1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The CYP7A1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
CYP7A1(Cytochrome P450 Subfamily VIIA Polypeptide 1), also called CYP7 or CHOLESTEROL 7-ALPHA-HYDROXYLASE, is an enzyme that in humans is encoded by the CYP7A1 gene. Using both mouse-human somatic cell hybrids and in situ chromosomal hybridization, Cohen et al.(1992) mapped the CYP7 gene to 8q11-q12. By transfection of reporter constructs, mutation analysis, and DNase footprinting, Molowa et al.(1992) identified areas of the CYP7A1 promoter region that showed hepatocyte-specific activation. They found HNF3 to be an activator of CYP7A1 activity. Agellon et al.(2002) found that wildtype mice and mice transgenic for human CYP7A1 respond differently to cholesterol feeding. Cholesterol feeding stimulated Cyp7a1 mRNA abundance and enzymatic activity in wildtype mice, but repressed human CYP7A1 mRNA and activity in transgenic mice.
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Related articles
1. Agellon, L. B., Drover, V. A. B., Cheema, S. K., Gbaguidi, G. F., Walsh, A. Dietary cholesterol fails to stimulate the human cholesterol 7-alpha-hydroxylase gene (CYP7A1) in transgenic mice. J. Biol. Chem. 277: 20131-20134, 2002. 2. Cohen, J. C., Cali, J. J., Jelinek, D. F., Mehrabian, M., Sparkes, R. S., Lusis, A. J., Russell, D. W., Hobbs, H. H. Cloning of the human cholesterol 7-alpha-hydroxylase gene (CYP7) and localization to chromosome 8q11-q12. Genomics 14: 153-161, 1992. 3. Molowa, D. T., Chen, W. S., Cimis, G. M., Tan, C. P. Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene. Biochemistry 31: 2539-2544, 1992.
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Gene Name
CYP7A1
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Protein Name
Cholesterol 7-alpha-monooxygenase
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Gene Full Name
cytochrome P450, family 7, subfamily A, polypeptide 1
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Synonyms
Cholesterol 7 alpha hydroxylase antibody|Cholesterol 7 alpha monooxygenase antibody|cholesterol 7 alpha-monooxygenase antibody|Cholesterol 7-alpha-hydroxylase antibody|Cholesterol 7-alpha-monooxygenase antibody|CP7A antibody|CP7A1_HUMAN antibody|CYP 7 antibody|CYP7 antibody|CYP7A1 antibody|CYPVII antibody|Cytochrome P450 7A1 antibody|cytochrome P450, family 7, subfamily A, polypeptide 1 antibody|Cytochrome P450, subfamily VIIA(cholesterol 7 alpha monooxygenase), antibody|cytochrome P450, subfamily VIIA(cholesterol 7 alpha-monooxygenase), antibody|Cytochrome P450, subfamily VIIA(cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody|MGC126826 antibody|MGC138389 antibody
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Uniprot ID
P22680
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
