Phospho-Cyclin E1 (Thr77) Antibody
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Catalog number
A1154-100
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Price
Please ask
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Size
100 µl
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Antibody Target
CCNE1
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Target Alternative Name
CCNE; G1/S-specific cyclin-E1
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Antibody Type
Polyclonal
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Species Reactivities
Human, Mouse
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Immunogen Sequence
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin E1
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Accession
P24864
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Gene Id
898
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol and 0.01% sodium azide
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Purification
Affinity purified
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Application
WB, IH, IF/IC
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Positive Control
WB: HEK293; IHC: human heart tissue; IFC: HEK293 cells
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Application And Usages
WB; 1:500 – 1:2000, IHC; 1:50 – 1:200, IF/IC; 1:50 – 1:100
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Description
Cyclin E1 is a regulatory subunit of the CDK2 kinase. It is central for the regulation of the G1/S transition and its abundance is tightly regulated throughout the cell cycle via ubiquitination. Cyclin E has been the subject of intense study in relation to tumorigenesis and cancer management and prognosis. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans
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Handling
The antibody solution should be gently mixed before use
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
Phospho-Cyclin E1 (Thr77) Antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
phosphorilated-Cyclin E1 (Thr77) (antibody to-)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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