Anti-ATF2 (Thr69) (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-12538R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  ATF2 Thr69
• Specificity
  This antibody reacts specifically with ATF2 (Thr69)
• Modification
  Phosphorylation
• Modification site
  Thr69
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Thr69
• Gene ID
  1386
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  ATF2 phospho T69; p-ATF2 phospho T69; ATF2 phospho Thr69 + Thr51; p-ATF2 phospho Thr69 + Thr51; CREB 2; HB 16;Activating Transcription Factor 2; ATF 2; Atf-2; ATF2 protein; cAMP Response Element Binding Protein 2; cAMP response element binding protein CRE BP1; cAMP-dependent transcription factor ATF-2
• Also known as
  ATF2 (Thr69) Polyclonal Antibody
• Other name
  Anti-ATF2 (Thr69) Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  ATF2   Thr69  
• Gene symbol
  GDNF, ATF2
• Short name
  Anti-ATF2 (Thr69)
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  ATF2 (Thr69) Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:4232
• Gene
  GDNF
• Long gene name
  glial cell derived neurotrophic factor
• Synonyms
  • ATF1
  • ATF2
  • HFB1-GDNF
• Synonyms name
  • astrocyte-derived trophic factor
  • glial cell line derived neurotrophic factor
  • glial derived neurotrophic factor
• Locus
  5p13.2
• Discovery year
  1995-05-04
• Entrez gene record
  2668
• Pubmed identfication
  • 8493557
• RefSeq identity
  • NM_000514
• Classification
  • GDNF family ligands
• VEGA ID
  OTTHUMG00000090809

Gene info

• Identity
  HGNC:784
• Gene
  ATF2
• Long gene name
  activating transcription factor 2
• Synonyms gene
  • CREB2
• Synonyms gene name
  • cAMP responsive element binding protein 2
• Synonyms
  • TREB7
  • CRE-BP1
  • HB16
• GenBank acession
  • X15875
• Locus
  2q31.1
• Discovery year
  1991-08-01
• Entrez gene record
  1386
• Pubmed identfication
  • 1833307
  • 1838349
• RefSeq identity
  • NM_001880
• Classification
  • Basic leucine zipper proteins
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000132424

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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